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frozen skno 1 cell pellets  (DSMZ)


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    Structured Review

    DSMZ frozen skno 1 cell pellets
    A) Venn diagram illustrating the overlap between human cell line SRA datasets and those containing anellovirus k-mers. B) Correlation analysis of SRA datasets with anellovirus k-mers hits, categorized by hit frequency: low (2-9 k-mers), medium (10-100 k-mers) and high (>100 k-mers). The dotted line connects each dataset to its corresponding number of hits. C) Classification of anellovirus k-mer-enriched SRA datasets by human cell line type. D) Overview of sequencing methodology for anellovirus <t>k-mer-rich</t> <t>SKNO-1</t> SRA datasets.
    Frozen Skno 1 Cell Pellets, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/frozen+skno+1+cell+pellets/bio_rxiv__64898__2026__01__22__701047-228-0-12?v=DSMZ
    Average 94 stars, based on 51 article reviews
    frozen skno 1 cell pellets - by Bioz Stars, 2026-07
    94/100 stars

    Images

    1) Product Images from "Integration of an anellovirus genome in the SKNO-1 acute myeloid leukemia cell line"

    Article Title: Integration of an anellovirus genome in the SKNO-1 acute myeloid leukemia cell line

    Journal: bioRxiv

    doi: 10.64898/2026.01.22.701047

    A) Venn diagram illustrating the overlap between human cell line SRA datasets and those containing anellovirus k-mers. B) Correlation analysis of SRA datasets with anellovirus k-mers hits, categorized by hit frequency: low (2-9 k-mers), medium (10-100 k-mers) and high (>100 k-mers). The dotted line connects each dataset to its corresponding number of hits. C) Classification of anellovirus k-mer-enriched SRA datasets by human cell line type. D) Overview of sequencing methodology for anellovirus k-mer-rich SKNO-1 SRA datasets.
    Figure Legend Snippet: A) Venn diagram illustrating the overlap between human cell line SRA datasets and those containing anellovirus k-mers. B) Correlation analysis of SRA datasets with anellovirus k-mers hits, categorized by hit frequency: low (2-9 k-mers), medium (10-100 k-mers) and high (>100 k-mers). The dotted line connects each dataset to its corresponding number of hits. C) Classification of anellovirus k-mer-enriched SRA datasets by human cell line type. D) Overview of sequencing methodology for anellovirus k-mer-rich SKNO-1 SRA datasets.

    Techniques Used: Sequencing


    Figure Legend Snippet:

    Techniques Used:

    A) Schematic representation of chromosome 21 in the SKNO-1 cell line, showing the integration site of the anellovirus genome (dotted green line). Red represents the centromere, blue indicates the repetitive region, and the grayscale sharing indicates Giemsa bands (darker shades correspond to higher heterochromatin and greater AT-rich regions). B) Detailed schematic of anellovirus integration within chromosome 21. The pink region denotes the anellovirus genome, with arrows indicating open reading frames (ORF). A grey arrow highlights ORF2 containing an early stop codon at position codon position 2, while an asterisk (*) marks the truncation of ORF1, preventing overlap with ORF3. The violet line highlights the duplicated sequences located at both termini of the viral genome, which differ by two-point mutations (C→A and G→A), while the blue region indicates an additional nucleotide segment corresponding to a MaLR-like element. C) Comparison between wild-type chromosome 12 and the chromosome 21 with the anellovirus genome integration. The integrant interrupts the RNA45SN2/RNA28SN2 rRNA gene. Dotted green lines indicate the region that has been altered within chromosome 21 including specific coordinates for the integration site. Below the schematic of chromosome 21 with the integration, PacBio long reads are shown (represented by grey rectangles), confirming the presence of the integrant in the SKNO-1 J subline cell.
    Figure Legend Snippet: A) Schematic representation of chromosome 21 in the SKNO-1 cell line, showing the integration site of the anellovirus genome (dotted green line). Red represents the centromere, blue indicates the repetitive region, and the grayscale sharing indicates Giemsa bands (darker shades correspond to higher heterochromatin and greater AT-rich regions). B) Detailed schematic of anellovirus integration within chromosome 21. The pink region denotes the anellovirus genome, with arrows indicating open reading frames (ORF). A grey arrow highlights ORF2 containing an early stop codon at position codon position 2, while an asterisk (*) marks the truncation of ORF1, preventing overlap with ORF3. The violet line highlights the duplicated sequences located at both termini of the viral genome, which differ by two-point mutations (C→A and G→A), while the blue region indicates an additional nucleotide segment corresponding to a MaLR-like element. C) Comparison between wild-type chromosome 12 and the chromosome 21 with the anellovirus genome integration. The integrant interrupts the RNA45SN2/RNA28SN2 rRNA gene. Dotted green lines indicate the region that has been altered within chromosome 21 including specific coordinates for the integration site. Below the schematic of chromosome 21 with the integration, PacBio long reads are shown (represented by grey rectangles), confirming the presence of the integrant in the SKNO-1 J subline cell.

    Techniques Used: Comparison

    Reads from ChIP-Seq SRA datasets were trimmed with fastp and aligned to a de-novo assembly of the anellovirus genome constructed from RNA-Seq data of the SKNO-1 cell line (see Methods). Alignment depth is plotted across the 3,425 bp reference genome. Plots are labeled with the associated SRA and ChIP-Seq experimental conditions (e.g. protein targeted, buffer). Predicted open reading frame coordinate intervals of the anellovirus assembly are displayed at the bottom. The span of the entire assembly is depicted by the black bar, and ORFs are shown as yellow boxes.
    Figure Legend Snippet: Reads from ChIP-Seq SRA datasets were trimmed with fastp and aligned to a de-novo assembly of the anellovirus genome constructed from RNA-Seq data of the SKNO-1 cell line (see Methods). Alignment depth is plotted across the 3,425 bp reference genome. Plots are labeled with the associated SRA and ChIP-Seq experimental conditions (e.g. protein targeted, buffer). Predicted open reading frame coordinate intervals of the anellovirus assembly are displayed at the bottom. The span of the entire assembly is depicted by the black bar, and ORFs are shown as yellow boxes.

    Techniques Used: ChIP-sequencing, Construct, RNA Sequencing, Labeling

    A) Maximum-likelihood phylogenetic tree of ORF1 nucleotide sequences from Alphatorquevirus, Betatorquevirus, and Gammatorquevirus reference genomes. Ultrafast bootstrap support values (1,000 replicates) are shown for key nodes. Anellovirus assembled from the SKNO-1 cell line is highlighted in red, and branches corresponding to the clade containing this virus together with sequences retrieved from NCBI BLAST showing >90% genomic identity are shown in pink. The Betatorquevirus clade is collapsed and represented as a green triangle, while the Gammatorquevirus clade is collapsed in blue. Branch lengths correspond to the number of substitutions per site. B) P-distance matrix comparing the SKNO-1 anellovirus, the SKNO-1–like anellovirus clade, and representative sequences of Alphatorquevirus homin27, homin28, and homin29. Bootstrap standard errors (100 replicates) are shown in italics.
    Figure Legend Snippet: A) Maximum-likelihood phylogenetic tree of ORF1 nucleotide sequences from Alphatorquevirus, Betatorquevirus, and Gammatorquevirus reference genomes. Ultrafast bootstrap support values (1,000 replicates) are shown for key nodes. Anellovirus assembled from the SKNO-1 cell line is highlighted in red, and branches corresponding to the clade containing this virus together with sequences retrieved from NCBI BLAST showing >90% genomic identity are shown in pink. The Betatorquevirus clade is collapsed and represented as a green triangle, while the Gammatorquevirus clade is collapsed in blue. Branch lengths correspond to the number of substitutions per site. B) P-distance matrix comparing the SKNO-1 anellovirus, the SKNO-1–like anellovirus clade, and representative sequences of Alphatorquevirus homin27, homin28, and homin29. Bootstrap standard errors (100 replicates) are shown in italics.

    Techniques Used: Virus

    A) Multiple alignment of amino acid sequence between Betatorquevirus LY1 ORF1 and Alphatorquevirus sp. isolate SKNO-1 ORF1 starting with ATG or ACG. The identical sites are marked as black. Betatorquevirus LY1 ORF1 is annotated with purple bar, and Alphatorquevirus sp. isolate SKNO-1 ORF1 is annotated with orange and yellow bar. B) The PDB structures of Betatorquevirus LY1 ORF1 and AlphaFold predicted structure Alphatorquevirus sp. isolate SKNO-1 ORF1 starting with ATG or ACG. The motif ARM, P2, P1, JR, and C-terminal are marked on the structure. Pentamers structures are shown on the right.
    Figure Legend Snippet: A) Multiple alignment of amino acid sequence between Betatorquevirus LY1 ORF1 and Alphatorquevirus sp. isolate SKNO-1 ORF1 starting with ATG or ACG. The identical sites are marked as black. Betatorquevirus LY1 ORF1 is annotated with purple bar, and Alphatorquevirus sp. isolate SKNO-1 ORF1 is annotated with orange and yellow bar. B) The PDB structures of Betatorquevirus LY1 ORF1 and AlphaFold predicted structure Alphatorquevirus sp. isolate SKNO-1 ORF1 starting with ATG or ACG. The motif ARM, P2, P1, JR, and C-terminal are marked on the structure. Pentamers structures are shown on the right.

    Techniques Used: Sequencing



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    frozen skno 1 cell pellets  (DSMZ)
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    DSMZ frozen skno 1 cell pellets
    A) Venn diagram illustrating the overlap between human cell line SRA datasets and those containing anellovirus k-mers. B) Correlation analysis of SRA datasets with anellovirus k-mers hits, categorized by hit frequency: low (2-9 k-mers), medium (10-100 k-mers) and high (>100 k-mers). The dotted line connects each dataset to its corresponding number of hits. C) Classification of anellovirus k-mer-enriched SRA datasets by human cell line type. D) Overview of sequencing methodology for anellovirus <t>k-mer-rich</t> <t>SKNO-1</t> SRA datasets.
    Frozen Skno 1 Cell Pellets, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/frozen+skno+1+cell+pellets/bio_rxiv__64898__2026__01__22__701047-228-0-12?v=DSMZ
    Average 94 stars, based on 1 article reviews
    frozen skno 1 cell pellets - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    A) Venn diagram illustrating the overlap between human cell line SRA datasets and those containing anellovirus k-mers. B) Correlation analysis of SRA datasets with anellovirus k-mers hits, categorized by hit frequency: low (2-9 k-mers), medium (10-100 k-mers) and high (>100 k-mers). The dotted line connects each dataset to its corresponding number of hits. C) Classification of anellovirus k-mer-enriched SRA datasets by human cell line type. D) Overview of sequencing methodology for anellovirus k-mer-rich SKNO-1 SRA datasets.

    Journal: bioRxiv

    Article Title: Integration of an anellovirus genome in the SKNO-1 acute myeloid leukemia cell line

    doi: 10.64898/2026.01.22.701047

    Figure Lengend Snippet: A) Venn diagram illustrating the overlap between human cell line SRA datasets and those containing anellovirus k-mers. B) Correlation analysis of SRA datasets with anellovirus k-mers hits, categorized by hit frequency: low (2-9 k-mers), medium (10-100 k-mers) and high (>100 k-mers). The dotted line connects each dataset to its corresponding number of hits. C) Classification of anellovirus k-mer-enriched SRA datasets by human cell line type. D) Overview of sequencing methodology for anellovirus k-mer-rich SKNO-1 SRA datasets.

    Article Snippet: Frozen SKNO-1 cell pellets (5 x 10 6 cells each) from the DSMZ and JCRB were submitted to the Translational Genomics Research Institute (TGen) for high-molecular-weight DNA extraction using the NEB Monarch HMW Extraction Kit for Cells & Blood.

    Techniques: Sequencing

    Journal: bioRxiv

    Article Title: Integration of an anellovirus genome in the SKNO-1 acute myeloid leukemia cell line

    doi: 10.64898/2026.01.22.701047

    Figure Lengend Snippet:

    Article Snippet: Frozen SKNO-1 cell pellets (5 x 10 6 cells each) from the DSMZ and JCRB were submitted to the Translational Genomics Research Institute (TGen) for high-molecular-weight DNA extraction using the NEB Monarch HMW Extraction Kit for Cells & Blood.

    Techniques:

    A) Schematic representation of chromosome 21 in the SKNO-1 cell line, showing the integration site of the anellovirus genome (dotted green line). Red represents the centromere, blue indicates the repetitive region, and the grayscale sharing indicates Giemsa bands (darker shades correspond to higher heterochromatin and greater AT-rich regions). B) Detailed schematic of anellovirus integration within chromosome 21. The pink region denotes the anellovirus genome, with arrows indicating open reading frames (ORF). A grey arrow highlights ORF2 containing an early stop codon at position codon position 2, while an asterisk (*) marks the truncation of ORF1, preventing overlap with ORF3. The violet line highlights the duplicated sequences located at both termini of the viral genome, which differ by two-point mutations (C→A and G→A), while the blue region indicates an additional nucleotide segment corresponding to a MaLR-like element. C) Comparison between wild-type chromosome 12 and the chromosome 21 with the anellovirus genome integration. The integrant interrupts the RNA45SN2/RNA28SN2 rRNA gene. Dotted green lines indicate the region that has been altered within chromosome 21 including specific coordinates for the integration site. Below the schematic of chromosome 21 with the integration, PacBio long reads are shown (represented by grey rectangles), confirming the presence of the integrant in the SKNO-1 J subline cell.

    Journal: bioRxiv

    Article Title: Integration of an anellovirus genome in the SKNO-1 acute myeloid leukemia cell line

    doi: 10.64898/2026.01.22.701047

    Figure Lengend Snippet: A) Schematic representation of chromosome 21 in the SKNO-1 cell line, showing the integration site of the anellovirus genome (dotted green line). Red represents the centromere, blue indicates the repetitive region, and the grayscale sharing indicates Giemsa bands (darker shades correspond to higher heterochromatin and greater AT-rich regions). B) Detailed schematic of anellovirus integration within chromosome 21. The pink region denotes the anellovirus genome, with arrows indicating open reading frames (ORF). A grey arrow highlights ORF2 containing an early stop codon at position codon position 2, while an asterisk (*) marks the truncation of ORF1, preventing overlap with ORF3. The violet line highlights the duplicated sequences located at both termini of the viral genome, which differ by two-point mutations (C→A and G→A), while the blue region indicates an additional nucleotide segment corresponding to a MaLR-like element. C) Comparison between wild-type chromosome 12 and the chromosome 21 with the anellovirus genome integration. The integrant interrupts the RNA45SN2/RNA28SN2 rRNA gene. Dotted green lines indicate the region that has been altered within chromosome 21 including specific coordinates for the integration site. Below the schematic of chromosome 21 with the integration, PacBio long reads are shown (represented by grey rectangles), confirming the presence of the integrant in the SKNO-1 J subline cell.

    Article Snippet: Frozen SKNO-1 cell pellets (5 x 10 6 cells each) from the DSMZ and JCRB were submitted to the Translational Genomics Research Institute (TGen) for high-molecular-weight DNA extraction using the NEB Monarch HMW Extraction Kit for Cells & Blood.

    Techniques: Comparison

    Reads from ChIP-Seq SRA datasets were trimmed with fastp and aligned to a de-novo assembly of the anellovirus genome constructed from RNA-Seq data of the SKNO-1 cell line (see Methods). Alignment depth is plotted across the 3,425 bp reference genome. Plots are labeled with the associated SRA and ChIP-Seq experimental conditions (e.g. protein targeted, buffer). Predicted open reading frame coordinate intervals of the anellovirus assembly are displayed at the bottom. The span of the entire assembly is depicted by the black bar, and ORFs are shown as yellow boxes.

    Journal: bioRxiv

    Article Title: Integration of an anellovirus genome in the SKNO-1 acute myeloid leukemia cell line

    doi: 10.64898/2026.01.22.701047

    Figure Lengend Snippet: Reads from ChIP-Seq SRA datasets were trimmed with fastp and aligned to a de-novo assembly of the anellovirus genome constructed from RNA-Seq data of the SKNO-1 cell line (see Methods). Alignment depth is plotted across the 3,425 bp reference genome. Plots are labeled with the associated SRA and ChIP-Seq experimental conditions (e.g. protein targeted, buffer). Predicted open reading frame coordinate intervals of the anellovirus assembly are displayed at the bottom. The span of the entire assembly is depicted by the black bar, and ORFs are shown as yellow boxes.

    Article Snippet: Frozen SKNO-1 cell pellets (5 x 10 6 cells each) from the DSMZ and JCRB were submitted to the Translational Genomics Research Institute (TGen) for high-molecular-weight DNA extraction using the NEB Monarch HMW Extraction Kit for Cells & Blood.

    Techniques: ChIP-sequencing, Construct, RNA Sequencing, Labeling

    A) Maximum-likelihood phylogenetic tree of ORF1 nucleotide sequences from Alphatorquevirus, Betatorquevirus, and Gammatorquevirus reference genomes. Ultrafast bootstrap support values (1,000 replicates) are shown for key nodes. Anellovirus assembled from the SKNO-1 cell line is highlighted in red, and branches corresponding to the clade containing this virus together with sequences retrieved from NCBI BLAST showing >90% genomic identity are shown in pink. The Betatorquevirus clade is collapsed and represented as a green triangle, while the Gammatorquevirus clade is collapsed in blue. Branch lengths correspond to the number of substitutions per site. B) P-distance matrix comparing the SKNO-1 anellovirus, the SKNO-1–like anellovirus clade, and representative sequences of Alphatorquevirus homin27, homin28, and homin29. Bootstrap standard errors (100 replicates) are shown in italics.

    Journal: bioRxiv

    Article Title: Integration of an anellovirus genome in the SKNO-1 acute myeloid leukemia cell line

    doi: 10.64898/2026.01.22.701047

    Figure Lengend Snippet: A) Maximum-likelihood phylogenetic tree of ORF1 nucleotide sequences from Alphatorquevirus, Betatorquevirus, and Gammatorquevirus reference genomes. Ultrafast bootstrap support values (1,000 replicates) are shown for key nodes. Anellovirus assembled from the SKNO-1 cell line is highlighted in red, and branches corresponding to the clade containing this virus together with sequences retrieved from NCBI BLAST showing >90% genomic identity are shown in pink. The Betatorquevirus clade is collapsed and represented as a green triangle, while the Gammatorquevirus clade is collapsed in blue. Branch lengths correspond to the number of substitutions per site. B) P-distance matrix comparing the SKNO-1 anellovirus, the SKNO-1–like anellovirus clade, and representative sequences of Alphatorquevirus homin27, homin28, and homin29. Bootstrap standard errors (100 replicates) are shown in italics.

    Article Snippet: Frozen SKNO-1 cell pellets (5 x 10 6 cells each) from the DSMZ and JCRB were submitted to the Translational Genomics Research Institute (TGen) for high-molecular-weight DNA extraction using the NEB Monarch HMW Extraction Kit for Cells & Blood.

    Techniques: Virus

    A) Multiple alignment of amino acid sequence between Betatorquevirus LY1 ORF1 and Alphatorquevirus sp. isolate SKNO-1 ORF1 starting with ATG or ACG. The identical sites are marked as black. Betatorquevirus LY1 ORF1 is annotated with purple bar, and Alphatorquevirus sp. isolate SKNO-1 ORF1 is annotated with orange and yellow bar. B) The PDB structures of Betatorquevirus LY1 ORF1 and AlphaFold predicted structure Alphatorquevirus sp. isolate SKNO-1 ORF1 starting with ATG or ACG. The motif ARM, P2, P1, JR, and C-terminal are marked on the structure. Pentamers structures are shown on the right.

    Journal: bioRxiv

    Article Title: Integration of an anellovirus genome in the SKNO-1 acute myeloid leukemia cell line

    doi: 10.64898/2026.01.22.701047

    Figure Lengend Snippet: A) Multiple alignment of amino acid sequence between Betatorquevirus LY1 ORF1 and Alphatorquevirus sp. isolate SKNO-1 ORF1 starting with ATG or ACG. The identical sites are marked as black. Betatorquevirus LY1 ORF1 is annotated with purple bar, and Alphatorquevirus sp. isolate SKNO-1 ORF1 is annotated with orange and yellow bar. B) The PDB structures of Betatorquevirus LY1 ORF1 and AlphaFold predicted structure Alphatorquevirus sp. isolate SKNO-1 ORF1 starting with ATG or ACG. The motif ARM, P2, P1, JR, and C-terminal are marked on the structure. Pentamers structures are shown on the right.

    Article Snippet: Frozen SKNO-1 cell pellets (5 x 10 6 cells each) from the DSMZ and JCRB were submitted to the Translational Genomics Research Institute (TGen) for high-molecular-weight DNA extraction using the NEB Monarch HMW Extraction Kit for Cells & Blood.

    Techniques: Sequencing